Article
International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant.
Analytical chemistry - 7 Feb 2017
Whale Alexandra S, Devonshire Alison S, Karlin-Neumann George, Regan Jack, Javier Leanne, Cowen Simon, Fernandez-Gonzalez Ana, Jones Gerwyn M, Redshaw Nicholas, Beck Julia, Berger Andreas W, Combaret Valérie, Dahl Kjersgaard Nina, Davis Lisa, Fina Frederic, Forshew Tim, Fredslund Andersen Rikke, Galbiati Silvia, González Hernández Álvaro, Haynes Charles A, Janku Filip, Lacave Roger, Lee Justin, Mistry Vilas, Pender Alexandra, Pradines Anne, Proudhon Charlotte, Saal Lao H, Stieglitz Elliot, Ulrich Bryan, Foy Carole A, Parkes Helen, Tzonev Svilen, Huggett Jim F
Abstract excerpt
This study tested the claim that digital PCR (dPCR) can offer highly reproducible quantitative measurements in disparate laboratories. Twenty-one laboratories measured four blinded samples containing different quantities of a KRAS fragment encoding G12D, an important genetic marker for guiding therapy of certain cancers. This marker is challenging to quantify reproducibly using quantitative PCR (qPCR) or next...
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