Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Improvement of digital PCR conditions for direct detection of KRAS mutations.

Journal of clinical laboratory analysis - 1 Aug 2020

Lee Jina, Kim Ji Hyun, Kang Sun Hyung, Yoo Hee Min

Abstract excerpt

BACKGROUND: In standard analytical conditions, an isolation step is essential for circulating tumor DNA (ctDNA) analysis. The necessity of this step becomes unclear with the development of highly sensitive detection methods. The aim of this study was to evaluate ctDNA mimetic nDNA detection as reference materials (RMs) using dPCR technologies either directly from serum or without serum. METHODS: To determine an...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Line, Tumor
Share to Circulating Tumor DNA
Share to Colorectal Neoplasms

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation

Share to Polymerase Chain Reaction

Share to Proto-Oncogene Proteins p21(ras)

Source

Open in PubMed Open full text

Identifiers

PMID: 32329932
DOI: 10.1002/jcla.23344
PMCID: PMC7439326

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Improvement of digital PCR conditions for direct detection of KRAS mutations.PMID 32329932Read ↗

Direct circulating tumor DNA detection from unpurified plasma using a digital PCR platform.Semantic proximity Read ↗

Evaluation of droplet digital PCR and next generation sequencing for characterizing DNA reference material for KRAS mutation detectionSemantic proximity Read ↗

[Detection of KRAS mutation in colorectal cancer patients' cfDNA with droplet digital PCR].Semantic proximity Read ↗

The diagnostic accuracy of digital PCR, ARMS and NGS for detecting KRAS mutation in cell-free DNA of patients with colorectal cancer: A protocol for systematic review and meta-analysis.Semantic proximity Read ↗

The diagnostic accuracy of digital PCR, ARMS and NGS for detecting KRAS mutation in cell-free DNA of patients with colorectal cancer: A systematic review and meta-analysis.Semantic proximity Read ↗

The evolution of molecular diagnosis using digital polymerase chain reaction to detect cancer via cell-free DNA and circulating tumor cells.Semantic proximity Read ↗

Circulating Tumor DNA Assays in Clinical Cancer ResearchSemantic proximity Read ↗

Circulating cell-free nucleic acids as biomarkers in colorectal cancer screening and diagnosis - an updateSemantic proximity Read ↗

Circulating tumor DNA in cancer diagnosis, monitoring, and prognosis.Semantic proximity Read ↗

Detection of Somatic Mutations with ddPCR from Liquid Biopsy of Colorectal Cancer Patients.Semantic proximity Read ↗

An improved digital polymerase chain reaction protocol to capture low-copy KRAS mutations in plasma cell-free DNA by resolving 'subsampling' issues.Semantic proximity Read ↗

Plasma vs. serum in circulating tumor DNA measurement: characterization by DNA fragment sizing and digital droplet polymerase chain reaction.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.