Article
Effects of the sequence and orientation of an expression cassette in tobacco transformed by dual Bt genes.
Plasmid - 1 Jan 2017
Qiu Tong, Dong Yan, Ren Yachao, Wang Jinmao, Yang Minsheng, Zhang Jun
Abstract excerpt
This study investigated the effects of the sequence arrangement and orientation of a target gene expression cassette in vectors on expression levels to determine the optimal combination for highly efficient multi-gene expression. Five plant transformation vectors were constructed using dual Bt genes, Cry1Ac and Cry3A, which differed in the sequence arrangement and orientation of the target gene expression...
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