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Chloroplast-targeted expression of the codon-optimized truncated cry1Ah gene in transgenic tobacco confers a high level of protection against insects.

Plant cell reports - 1 Aug 2013

Li Xiuying, Li Shengyan, Lang Zhihong, Zhang Jie, Zhu Li, Huang Dafang

Abstract excerpt

KEY MESSAGE: The study of insect-resistant transgenic tobacco provides a good foundation for the further application of the cry1Ah gene in other important crops. To improve transgene expression levels and insect resistance, the coding sequence of the novel Bacillus thuringiensis insecticidal gene cry1Ah (truncated cry1Ah) was modified according to the codon bias of the plant by increasing its GC content from the...

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PMID: 23620344
DOI: 10.1007/s00299-013-1444-z

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