Article
A super twin T-DNA vector that allows independent gene expression during Agrobacterium-mediated transformation.
Plasmid - 1 Jan 2000
Yang Qiang, Deng Mei, Zhang Ling-Ling, Zhang Xiao-Wei, Wang Le-Ning, Chen Hu, Ma Jian, Qi Peng-Fei, Jiang Qian-Tao, Lan Xiu-Jin, Wei Yu-Ming, Zheng You-Liang
Abstract excerpt
In this study, we designed and constructed a super twin T-DNA vector (pTRIDT313-g) containing two independent T-DNA cassettes-one for the selection gene Hyg and the other for the target gene Gus-to produce marker-free transgenic lines. The resulting vector was transformed into tobacco, and polymerase chain reaction (PCR) analysis showed four types of gene combinations in the T1 and T2 generations: Gus only, Hyg...
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