Article
Autoantibody to scaffold attachment factor B (SAFB): A novel connective tissue disease-related autoantibody associated with interstitial lung disease.
Journal of autoimmunity - 1 Jan 2017
Takeuchi Akiko, Matsushita Takashi, Kaji Kenzo, Okamoto Yoshinobu, Yasui Masahide, Hirata Masayoshi, Oishi Naoto, Higashi Akira, Seishima Mariko, Asano Tomoya, Fujimoto Manabu, Kuwana Masataka, Takehara Kazuhiko, Hamaguchi Yasuhito
Abstract excerpt
OBJECTIVE: To identify and characterize a novel connective tissue disease (CTD)-related autoantibody (autoAb) directed against scaffold attachment factor B (SAFB). METHODS: AutoAb specificity was analyzed using RNA and protein-immunoprecipitation assays. Autoimmune targets were affinity purified using patients' sera and subjected to liquid chromatography mass spectrometry. RESULTS: By immunoprecipitation assay,...
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