Article
NGS-based reverse genetic screen for common embryonic lethal mutations compromising fertility in livestock.
Genome research - 1 Oct 2016
Charlier Carole, Li Wanbo, Harland Chad, Littlejohn Mathew, Coppieters Wouter, Creagh Frances, Davis Steve, Druet Tom, Faux Pierre, Guillaume François, Karim Latifa, Keehan Mike, Kadri Naveen Kumar, Tamma Nico, Spelman Richard, Georges Michel
Abstract excerpt
We herein report the result of a large-scale, next generation sequencing (NGS)-based screen for embryonic lethal (EL) mutations in Belgian beef and New Zealand dairy cattle. We estimated by simulation that cattle might carry, on average, ∼0.5 recessive EL mutations. We mined exome sequence data from >600 animals, and identified 1377 stop-gain, 3139 frame-shift, 1341 splice-site, 22,939 disruptive missense, 62,399...
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