Article
A computational protocol to evaluate the effects of protein mutants in the kinase gatekeeper position on the binding of ATP substrate analogues.
BMC research notes - 20 Feb 2017
Romano Valentina, de Beer Tjaart A P, Schwede Torsten
Abstract excerpt
BACKGROUND: The determination of specific kinase substrates in vivo is challenging due to the large number of protein kinases in cells, their substrate specificity overlap, and the lack of highly specific inhibitors. In the late 90s, Shokat and coworkers developed a protein engineering-based method addressing the question of identification of substrates of protein kinases. The approach was based on the...
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