Article
Recombinant herpes simplex virus type 1 strains with targeted mutations relevant for aciclovir susceptibility.
Scientific reports - 18 Jul 2016
Brunnemann Anne-Kathrin, Liermann Kristin, Deinhardt-Emmer Stefanie, Maschkowitz Gregor, Pohlmann Anja, Sodeik Beate, Fickenscher Helmut, Sauerbrei Andreas, Krumbholz Andi
Abstract excerpt
Here, we describe a novel reliable method to assess the significance of individual mutations within the thymidine kinase (TK) gene of herpes simplex virus type 1 (HSV-1) to nucleoside analogue resistance. Eleven defined single nucleotide polymorphisms that occur in the TK gene of clinical HSV-1 isolates and a fluorescence reporter were introduced into the HSV-1 strain 17(+) that had been cloned into a bacterial...
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