Article
Enhancement of a high efficient autoinducible expression system in Bacillus subtilis by promoter engineering.
Protein expression and purification - 1 Nov 2016
Cheng Jintao, Guan Chengran, Cui Wenjing, Zhou Li, Liu Zhongmei, Li Weijiang, Zhou Zhemin
Abstract excerpt
Quorum-sensing related promoter srfA (PsrfA) was used to construct autoinducible expression system for production of recombinant proteins in Bacillus subtilis. PsrfA was prominent in the unique property of inducer-free activity that is closely correlated with cell density. Here, using green fluorescent protein (GFP) as the reporter protein, PsrfA was optimized by shortening its sequences and changing the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
