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Simultaneous purification of DNA and RNA from microbiota in a single colonic mucosal biopsy

27 Jun 2016

Abstract excerpt

BACKGROUND: Nucleic acid purification methods are of importance when performing microbiota studies and especially when analysing the intestinal microbiota as we here find a wide range of different microbes. Various considerations must be taken to lyse the microbial cell wall of each microbe. In the present article, we compare several tissue lysis steps and commercial purification kits, to achieve a joint RNA and...

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PMID: 27352784
DOI: 10.1186/s13104-016-2110-7

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