Article
Triggering the ExoS regulon of Pseudomonas aeruginosa: A GFP-reporter analysis of exoenzyme (Exo) S, ExoT and ExoU synthesis.
Microbial pathogenesis - 1 Dec 2000
Hornef M W, Roggenkamp A, Geiger A M, Hogardt M, Jacobi C A, Heesemann J
Abstract excerpt
The ExoS regulon of Pseudomonas aeruginosa encodes diverse type III secreted effector proteins which have been shown to exert cytotoxic effects in cell culture experiments. However, little information exists about the environmental conditions and stimuli for upregulation of the ExoS regulon. Translational reporter fusion proteins of exoenzyme (Exo) S, ExoT and ExoU, as well as the type II secreted exotoxin A...
Topics
- ADP Ribose Transferases
- Bacterial Proteins
- Bacterial Toxins
- Blotting, Southern
- Coculture Techniques
- Conjugation, Genetic
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Exotoxins
- Flow Cytometry
- Green Fluorescent Proteins
- HeLa Cells
- Histidine Kinase