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Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Rufián José S, López-Márquez Diego, López-Pagán Nieves, Grant Murray, Ruiz-Albert Javier, Beuzón Carmen R

Abstract excerpt

The last decade has seen significant effort directed toward the role of phenotypic heterogeneity in bacterial adaptation. Phenotypic heterogeneity usually refers to phenotypic diversity that takes place through nongenetic means, independently of environmental induced variation. Recent findings are changing how microbiologists analyze bacterial behavior, with a shift from traditional assays averaging large...

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PMID: 29288455
DOI: 10.1007/978-1-4939-7604-1_15

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