Article
megasat: automated inference of microsatellite genotypes from sequence data.
Molecular ecology resources - 1 Mar 2017
Zhan Luyao, Paterson Ian G, Fraser Bonnie A, Watson Beth, Bradbury Ian R, Nadukkalam Ravindran Praveen, Reznick David, Beiko Robert G, Bentzen Paul
Abstract excerpt
megasat is software that enables genotyping of microsatellite loci using next-generation sequencing data. Microsatellites are amplified in large multiplexes, and then sequenced in pooled amplicons. megasat reads sequence files and automatically scores microsatellite genotypes. It uses fuzzy matches to allow for sequencing errors and applies decision rules to account for amplification artefacts, including...
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