Article
Sequence-Specific Protein Aggregation Generates Defined Protein Knockdowns in Plants.
Plant physiology - 1 Jun 2016
Betti Camilla, Vanhoutte Isabelle, Coutuer Silvie, De Rycke Riet, Mishev Kiril, Vuylsteke Marnik, Aesaert Stijn, Rombaut Debbie, Gallardo Rodrigo, De Smet Frederik, Xu Jie, Van Lijsebettens Mieke, Van Breusegem Frank, Inzé Dirk, Rousseau Frederic, Schymkowitz Joost, Russinova Eugenia
Abstract excerpt
Protein aggregation is determined by short (5-15 amino acids) aggregation-prone regions (APRs) of the polypeptide sequence that self-associate in a specific manner to form β-structured inclusions. Here, we demonstrate that the sequence specificity of APRs can be exploited to selectively knock down proteins with different localization and function in plants. Synthetic aggregation-prone peptides derived from the...
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