Article
Mapping-by-sequencing in complex polyploid genomes using genic sequence capture: a case study to map yellow rust resistance in hexaploid wheat.
The Plant journal : for cell and molecular biology - 1 Aug 2016
Gardiner Laura-Jayne, Bansept-Basler Pauline, Olohan Lisa, Joynson Ryan, Brenchley Rachel, Hall Neil, O'Sullivan Donal M, Hall Anthony
Abstract excerpt
Previously we extended the utility of mapping-by-sequencing by combining it with sequence capture and mapping sequence data to pseudo-chromosomes that were organized using wheat-Brachypodium synteny. This, with a bespoke haplotyping algorithm, enabled us to map the flowering time locus in the diploid wheat Triticum monococcum L. identifying a set of deleted genes (Gardiner et al., 2014). Here, we develop this...
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