Article
RNA-Seq bulked segregant analysis enables the identification of high-resolution genetic markers for breeding in hexaploid wheat.
Plant biotechnology journal - 1 Jun 2015
Ramirez-Gonzalez Ricardo H, Segovia Vanesa, Bird Nicholas, Fenwick Paul, Holdgate Sarah, Berry Simon, Jack Peter, Caccamo Mario, Uauy Cristobal
Abstract excerpt
The identification of genetic markers linked to genes of agronomic importance is a major aim of crop research and breeding programmes. Here, we identify markers for Yr15, a major disease resistance gene for wheat yellow rust, using a segregating F2 population. After phenotyping, we implemented RNA sequencing (RNA-Seq) of bulked pools to identify single-nucleotide polymorphisms (SNP) associated with Yr15. Over...
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