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Surface display vectors for selective detection and isolation of high level antibody producing cells

4 May 2016

Abstract excerpt

Cell line generation for production of biopharmaceuticals in mammalian cells usually involves intensive screening of clones to identify the rare high producers. In order to facilitate efficient and selective fluorescence activated cell sorting (FACS) based enrichment and cloning of antibody producing CHO cells, we developed a special vector setup by inserting a leaky translation termination signal between the...

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PMID: 27144878
DOI: 10.1002/bit.26000

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