Article
A Novel Luciferase Assay For Sensitively Monitoring Myocilin Variants in Cell Culture.
Investigative ophthalmology & visual science - 1 Apr 2016
Zadoo Serena, Nguyen Annie, Zode Gulab, Hulleman John D
Abstract excerpt
PURPOSE: Primary open angle glaucoma-associated mutations in myocilin (MYOC) cause protein "nonsecretion," rendering secreted MYOC difficult to detect using conventional techniques. This study focused on developing and using an assay that can quickly and easily detect mutant MYOC secretion. METHODS: We fused Gaussia luciferase (eGLuc2) to MYOC variants and expressed the constructs in HEK-293T and NTM-5 cells....
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