Article
Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin.
Journal of cell science - 15 May 2016
Kubo Tomohiro, Brown Jason M, Bellve Karl, Craige Branch, Craft Julie M, Fogarty Kevin, Lechtreck Karl F, Witman George B
Abstract excerpt
The assembly and maintenance of most cilia and flagella rely on intraflagellar transport (IFT). Recent in vitro studies have suggested that, together, the calponin-homology domain within the IFT81 N-terminus and the highly basic N-terminus of IFT74 form a module for IFT of tubulin. By using Chlamydomonas mutants for IFT81 and IFT74, we tested this hypothesis in vivo. Modification of the predicted tubulin-binding...
Topics
- Biological Transport
- Carrier Proteins
- Chlamydomonas reinhardtii
- Cilia
- Flagella
- Phenotype
- Protein Binding
- Tubulin