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Article

Probing phenotypic growth in expanding Bacillus subtilis biofilms.

Applied microbiology and biotechnology - 1 May 2016

Wang Xiaoling, Koehler Stephan A, Wilking James N, Sinha Naveen N, Cabeen Matthew T, Srinivasan Siddarth, Seminara Agnese, Rubinstein Shmuel, Sun Qingping, Brenner Michael P, Weitz David A

Abstract excerpt

We develop an optical imaging technique for spatially and temporally tracking biofilm growth and the distribution of the main phenotypes of a Bacillus subtilis strain with a triple-fluorescent reporter for motility, matrix production, and sporulation. We develop a calibration procedure for determining the biofilm thickness from the transmission images, which is based on Beer-Lambert's law and involves...

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PMID: 27003268
DOI: 10.1007/s00253-016-7461-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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