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Article

Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging.

Journal of proteome research - 3 Jun 2016

Si Tong, Li Bin, Zhang Ke, Xu Yiran, Zhao Huimin, Sweedler Jonathan V

Abstract excerpt

Colony biofilms of Bacillus subtilis are a widely used model for studying cellular differentiation. Here, we applied matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to examine cellular and molecular heterogeneity in B. subtilis colony biofilms. From B. subtilis cells cultivated on a biofilm-promoting medium, we detected two cannibalistic factors not found in previous MALDI MSI...

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Identifiers

PMID: 27136705
DOI: 10.1021/acs.jproteome.6b00127
PMCID: PMC4895246

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