Article
Rapid gene identification in sugar beet using deep sequencing of DNA from phenotypic pools selected from breeding panels.
BMC genomics - 15 Mar 2016
Ries David, Holtgräwe Daniela, Viehöver Prisca, Weisshaar Bernd
Abstract excerpt
BACKGROUND: The combination of bulk segregant analysis (BSA) and next generation sequencing (NGS), also known as mapping by sequencing (MBS), has been shown to significantly accelerate the identification of causal mutations for species with a reference genome sequence. The usual approach is to cross homozygous parents that differ for the monogenic trait to address, to perform deep sequencing of DNA from F2 plants...
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