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Article

High throughput gene complementation screening permits identification of a mammalian mitochondrial protein synthesis (ρ(-)) mutant.

Biochimica et biophysica acta - 1 Aug 2016

Potluri Prasanth, Procaccio Vincent, Scheffler Immo E, Wallace Douglas C

Abstract excerpt

To identify nuclear DNA (nDNA) oxidative phosphorylation (OXPHOS) gene mutations using cultured cells, we have developed a complementation system based on retroviral transduction with a full length cDNA expression library and selection for OXHOS function by growth in galactose. We have used this system to transduce the Chinese hamster V79-G7 OXPHOS mutant cell line with a defect in mitochondrial protein...

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Identifiers

PMID: 26946086
DOI: 10.1016/j.bbabio.2016.02.021
PMCID: PMC4893908

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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