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Establishment of a confluent monolayer model with human primary trophoblast cells: novel insights into placental glucose transport

29 Feb 2016

Abstract excerpt

STUDY HYPOTHESIS: Using optimized conditions, primary trophoblast cells isolated from human term placenta can develop a confluent monolayer in vitro, which morphologically and functionally resembles the microvilli structure found in vivo. STUDY FINDING: We report the successful establishment of a confluent human primary trophoblast monolayer using pre-coated polycarbonate inserts, where the integrity and...

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PMID: 26931579
DOI: 10.1093/molehr/gaw018

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