Article
Development of an improved reverse genetics system for Akabane bunyavirus.
Journal of virological methods - 1 Jun 2016
Takenaka-Uema Akiko, Sugiura Keita, Bangphoomi Norasuthi, Shioda Chieko, Uchida Kazuyuki, Kato Kentaro, Haga Takeshi, Murakami Shin, Akashi Hiroomi, Horimoto Taisuke
Abstract excerpt
Akabane disease, caused by the insect-transmitted Akabane virus (AKAV), affects livestock by causing life-threatening deformities or mortality of fetuses. Therefore, Akabane disease has led to notable economic losses in numerous countries, including Japan. In this short communication, a new T7 RNA polymerase-based AKAV reverse genetics system was developed. Using this system, in which three plasmids transcribing...
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