Article
Rapid identification of causative insertions underlying Medicago truncatula Tnt1 mutants defective in symbiotic nitrogen fixation from a forward genetic screen by whole genome sequencing.
BMC genomics - 27 Feb 2016
Veerappan Vijaykumar, Jani Mehul, Kadel Khem, Troiani Taylor, Gale Ronny, Mayes Tyler, Shulaev Elena, Wen Jiangqi, Mysore Kirankumar S, Azad Rajeev K, Dickstein Rebecca
Abstract excerpt
BACKGROUND: In the model legume Medicago truncatula, the near saturation genome-wide Tnt1 insertion mutant population in ecotype R108 is a valuable tool in functional genomics studies. Forward genetic screens have identified many Tnt1 mutants defective in nodule development and symbiotic nitrogen fixation (SNF). However, progress toward identifying the causative mutations of these symbiotic mutants has been slow...
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