Article
An efficient reverse genetics platform in the model legume Medicago truncatula.
The New phytologist - 1 Feb 2014
Cheng Xiaofei, Wang Mingyi, Lee Hee-Kyung, Tadege Million, Ratet Pascal, Udvardi Michael, Mysore Kirankumar S, Wen Jiangqi
Abstract excerpt
Medicago truncatula is one of the model species for legume studies. In an effort to develop legume genetics resources, > 21 700 Tnt1 retrotransposon insertion lines have been generated. To facilitate fast-growing needs in functional genomics, two reverse genetics approaches have been established: web-based database searching and PCR-based reverse screening. More than 840 genes have been reverse screened using the...
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