Article
Identification of Suitable Endogenous Normalizers for qRT-PCR Analysis of Plasma microRNA Expression in Essential Hypertension.
Molecular biotechnology - 1 Mar 2016
Solayman Mohamed Hassan M, Langaee Taimour, Patel Archanakumari, El-Wakeel Lamia, El-Hamamsy Manal, Badary Osama, Johnson Julie A
Abstract excerpt
Circulating microRNAs (miRNAs) are promising biomarkers for many diseases. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a gold standard for miRNA expression profiling that requires proper data normalization. Since there is no universal normalizer, it is recommended to evaluate normalizers under every experimental condition. This study describes the identification of suitable...
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