Article

Validation of reference genes for the normalization of the RT- qPCR in peripheral blood mononuclear cells of septic patients

2022-08-24

Abstract excerpt

<h4>Objective: </h4> To screen and validate reference genes suitable for gene mRNA expression study in peripheral blood mononuclear cells (PBMCs) between septic patients and healthy controls (HC). <h4>Methods: </h4> Total RNA in PBMCs was extracted and RT-qPCR was used to determine the mRNA expression profiles of 9 candidate genes, including ACTB, B2M, GAPDH, GUSB, HPRT1, PGK1, RPL13A, SDHA and YWHAZ. The genes ex...

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Literature Corpus work: 3c6df769-2b8a-5f17-b29b-271c3536e762
DOI: 10.21203/rs.3.rs-1941625/v1

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