Article
A multiplex real-time PCR assay targeting virulence and resistance genes in Salmonella enterica serotype Typhimurium.
BMC microbiology - 27 Jun 2011
Bugarel Marie, Granier Sophie A, Weill François-Xavier, Fach Patrick, Brisabois Anne
Abstract excerpt
BACKGROUND: Typhimurium is the main serotype of Salmonella enterica subsp. enterica implicated in food-borne diseases worldwide. This study aimed to detect the prevalence of ten markers combined in a macro-array based on multiplex real-time PCR. We targeted characteristic determinants located on pathogenicity islands (SPI-2 to -5, virulence plasmid pSLT and Salmonella genomic island 1 (SGI1)) as well as a...
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