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Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity

14 Dec 2015

Abstract excerpt

The tentative clinical application of human pluripotent stem cells (hPSCs), such as human embryonic stem cells and human induced pluripotent stem cells, is restricted by the possibility of xenogenic contamination resulting from the use of mouse embryonic fibroblasts (MEFs) as a feeder layer. Therefore, we investigated hPSC cultures on biomaterials with different elasticities that were grafted with different...

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PMID: 26656754
DOI: 10.1038/srep18136

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