Article
Next-Generation Sequencing Workflow for NSCLC Critical Samples Using a Targeted Sequencing Approach by Ion Torrent PGM™ Platform.
International journal of molecular sciences - 3 Dec 2015
Vanni Irene, Coco Simona, Truini Anna, Rusmini Marta, Dal Bello Maria Giovanna, Alama Angela, Banelli Barbara, Mora Marco, Rijavec Erika, Barletta Giulia, Genova Carlo, Biello Federica, Maggioni Claudia, Grossi Francesco
Abstract excerpt
Next-generation sequencing (NGS) is a cost-effective technology capable of screening several genes simultaneously; however, its application in a clinical context requires an established workflow to acquire reliable sequencing results. Here, we report an optimized NGS workflow analyzing 22 lung cancer-related genes to sequence critical samples such as DNA from formalin-fixed paraffin-embedded (FFPE) blocks and...
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