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Article

Characterization of Aspartate Kinase from Corynebacterium pekinense and the Critical Site of Arg169.

International journal of molecular sciences - 27 Nov 2015

Min Weihong, Li Huiying, Li Hongmei, Liu Chunlei, Liu Jingsheng

Abstract excerpt

Aspartate kinase (AK) is the key enzyme in the biosynthesis of aspartate-derived amino acids. Recombinant AK was efficiently purified and systematically characterized through analysis under optimal conditions combined with steady-state kinetics study. Homogeneous AK was predicted as a decamer with a molecular weight of ~48 kDa and a half-life of 4.5 h. The enzymatic activity was enhanced by ethanol and Ni(2+)....

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Identifiers

PMID: 26633359
DOI: 10.3390/ijms161226098
PMCID: PMC4691045

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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