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Article

The importance of start codon of nosM in nosiheptide production.

Chinese journal of natural medicines - 1 Nov 2015

Jiang Lei, Xue Yan-Jiu, Liu Wei-Ying, Ma Min, Wu Xu-Ri, Wang Shu-Zhen, Chen Yi-Jun

Abstract excerpt

The present study was designed to investigate the effects of start codon of nosM on the biosynthesis of nosiheptide. Target genes were amplified by overlap PCR. After homologous recombination to construct engineered strains, nosiheptide production was analyzed by HPLC. Three mutants with different start codon of nosM were constructed, and nosiheptide production of each mutant was analyzed and compared....

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PMID: 26614460
DOI: 10.1016/S1875-5364(15)30089-3

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