Article
The Tomato Nucleotide-binding Leucine-rich Repeat Immune Receptor I-2 Couples DNA-binding to Nucleotide-binding Domain Nucleotide Exchange.
The Journal of biological chemistry - 15 Jan 2016
Fenyk Stepan, Dixon Christopher H, Gittens William H, Townsend Philip D, Sharples Gary J, Pålsson Lars-Olof, Takken Frank L W, Cann Martin J
Abstract excerpt
Plant nucleotide-binding leucine-rich repeat (NLR) proteins enable plants to recognize and respond to pathogen attack. Previously, we demonstrated that the Rx1 NLR of potato is able to bind and bend DNA in vitro. DNA binding in situ requires its genuine activation following pathogen perception. However, it is unknown whether other NLR proteins are also able to bind DNA. Nor is it known how DNA binding relates to...
Topics
- Adenosine Diphosphate
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Amino Acid Substitution
- Binding Sites
- DNA, Single-Stranded
- DNA-Binding Proteins
- Electrophoretic Mobility Shift Assay
- Hydrolysis
- Leucine-Rich Repeat Proteins
- Solanum lycopersicum
- Models, Molecular