Article
[HRM identification of Chinese medicinal materials Mutong].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Jun 2015
Hu Jun, Zhan Zhi-lai, Yuan Yuan, Huang Lu-qi, Liu Yong
Abstract excerpt
High resolution melting (HRM), an important technology for genotyping and mutation scanning, has broad prospects in the authentification of traditional Chinese medicine. This paper selected universal trnH-psbA primers and used HRM to establish a new methods for identification of Akebia herbs. PCR was conduct at the annealing temperature of 58 degrees C and 35 cycles. The range of the DNA template concentration,...
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