Development of a duplex Proofman ladder-shape melting temperature isothermal amplification assay for simultaneous discrimination of Schisandra chinensis and Schisandra sphenanthera using species-specific ITS1 SNPs.

Abstract excerpt

Traditional Chinese medicine (TCM) products frequently face adulteration risks, demanding rapid, field-deployable authentication methods. We developed a novel duplex Proofman ladder-shape melting temperature isothermal amplification (Proofman-duplex-LMTIA) assay that simultaneously discriminates Schisandra chinensis (authentic ingredient) from Schisandra sphenanthera (common adulterant) by targeting...

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