Article
[Molecular identification of hairy antler by analysis of high resolution melting].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Feb 2015
Chen Kang, Jiang Chao, Yuan Yuan, Huang Lu-qi, Jin Yan
Abstract excerpt
High resolution melting (HRM) , an important technology for genotyping and mutation scanning, has broad prospects in the authenticity of traditional Chinese medicine. This paper selected universal CO I primers and used HRM to establish a new method for authenticity of Hairy Antler. PCR was conducted at the annealing temperature of 60 °C and 45 cycles. The range of the DNA template concentration, the primer...
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