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Article

[Allele-specific PCR to identification of Achyranthis Bidentatae Radix and Cyathulae Radix formula granules].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Mar 2018

Meng Hu-Biao, Yuan Yuan, Liu Fu-Yan, Jiang Chao, Jin Yan, Zhao Yu-Yang

Abstract excerpt

To establish a robust and accuracy molecular method to identify Achyranthis Bidentatae Radix and Cyathulae Radix formula granules. ITS sequences of Achyranthes bidentata and Cyathula officinalis were aligned, specific SNPs (single nucleotide polymorphisms) were excavated, specific primers were designed and allele-specific PCR method was established. The genomic DNA was successfully extracted from the herbal...

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PMID: 29676092
DOI: 10.19540/j.cnki.cjcmm.20180110.005

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