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Article

Using Next Generation RAD Sequencing to Isolate Multispecies Microsatellites for Pilosocereus (Cactaceae).

PloS one - 1 Jan 2015

Bonatelli Isabel A S, Carstens Bryan C, Moraes Evandro M

Abstract excerpt

Microsatellite markers (also known as SSRs, Simple Sequence Repeats) are widely used in plant science and are among the most informative molecular markers for population genetic investigations, but the development of such markers presents substantial challenges. In this report, we discuss how next generation sequencing can replace the cloning, Sanger sequencing, identification of polymorphic loci, and testing...

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Identifiers

PMID: 26561396
DOI: 10.1371/journal.pone.0142602
PMCID: PMC4641700

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