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A rapid and cost-effective approach for the development of polymorphic microsatellites in non-model species using paired-end RAD sequencing.

Molecular genetics and genomics : MGG - 1 Oct 2017

Xue Dong-Xiu, Li Yu-Long, Liu Jin-Xian

Abstract excerpt

As one of the most informative and versatile DNA-based markers, microsatellites have been widely used in population and conservation genetic studies. However, the development of microsatellites has traditionally been laborious, time-consuming, and expensive. In the present study, a rapid and cost-effective "RAD-seq-Assembly-Microsatellite" approach was developed to identify abundant microsatellite markers in...

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DNA Primers
Genetic Markers
Genetics, Population
High-Throughput Nucleotide Sequencing
Microsatellite Repeats

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymorphism, Single Nucleotide

Sequence Analysis, DNA

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PMID: 28634825
DOI: 10.1007/s00438-017-1337-x

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