Article
Ultra-barcoding in cacao (Theobroma spp.; Malvaceae) using whole chloroplast genomes and nuclear ribosomal DNA.
American journal of botany - 1 Feb 2012
Kane Nolan, Sveinsson Saemundur, Dempewolf Hannes, Yang Ji Yong, Zhang Dapeng, Engels Johannes M M, Cronk Quentin
Abstract excerpt
PREMISE OF STUDY: To reliably identify lineages below the species level such as subspecies or varieties, we propose an extension to DNA-barcoding using next-generation sequencing to produce whole organellar genomes and substantial nuclear ribosomal sequence. Because this method uses much longer versions of the traditional DNA-barcoding loci in the plastid and ribosomal DNA, we call our approach ultra-barcoding...
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