Article
Characterization of Novel Microsatellite Loci for Primula poissonii (Primulaceae) Using High-Throughput Sequencing Technology.
Molecules (Basel, Switzerland) - 9 May 2016
Liu Yun-Jiao, Zhang Cai-Yun, Hao Gang, Ge Xue-Jun, Yan Hai-Fei
Abstract excerpt
Primula poissonii (Primulaceae) is a perennial herb, widely distributed in the Hengduan Mountain region of Southwest China. In this study, Roche 454 pyrosequencing was used to isolate microsatellite markers. A total of 4528 unique sequences were identified from 68,070 unique reads. Of these, eighty-seven microsatellite loci were screened for utility using two criteria: successful PCR amplification and variation...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
