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Article

Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation.

Molecular medicine reports - 1 Jan 2016

Wang Xiaoxu, Li Shiyu, Lu Xiaoran, Hu Pan, Chen Haiyan, Li Zheng, Bu Zhaoyang, Lang Xulong, Wang Xinglong

Abstract excerpt

Rapid and efficient inactivation of a target gene in Escherichia coli chromosomes is required to investigate metabolic engineering. In the present study, a multiple gene inactivation approach was demonstrated in four strains of enterotoxigenic E. coli (ETEC), which are the predominant pathogenic bacteria causing piglet diarrhea, mediated by λ Red and Xer recombination. The chromosomal genes, luxS and pfs were...

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PMID: 26549009
DOI: 10.3892/mmr.2015.4532

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