Article
Effect of serial cell passaging in the retention of fiber type and mitochondrial content in primary human myotubes.
Obesity (Silver Spring, Md.) - 1 Dec 2015
Covington Jeffrey D, Myland Cassandra K, Rustan Arild C, Ravussin Eric, Smith Steven R, Bajpeyi Sudip
Abstract excerpt
OBJECTIVE: The purpose of the study was to determine the effects of passaging on retention of donor phenotypic characteristics in primary human myotubes. METHODS: Primary muscle cultures and serial passaged myotubes from physically active, sedentary lean, and individuals with type 2 diabetes were established. Maximal ATP synthesis capacity (ATPmax) and resting ATP flux (ATPase) in vivo were measured by (31) P...
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