Article
Plaque2.0-A High-Throughput Analysis Framework to Score Virus-Cell Transmission and Clonal Cell Expansion.
PloS one - 1 Jan 2015
Yakimovich Artur, Andriasyan Vardan, Witte Robert, Wang I-Hsuan, Prasad Vibhu, Suomalainen Maarit, Greber Urs F
Abstract excerpt
Classical plaque assay measures the propagation of infectious agents across a monolayer of cells. It is dependent on cell lysis, and limited by user-specific settings and low throughput. Here, we developed Plaque2.0, a broadly applicable, fluorescence microscopy-based high-throughput method to mine patho-biological clonal cell features. Plaque2.0 is an open source framework to extract information from chemically...
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