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Sensitive detection of Porcine circovirus-2 by droplet digital polymerase chain reaction

21 Sept 2015

Abstract excerpt

Sensitive detection of Porcine circovirus-2 (PCV-2) is very important for surveillance of postweaning multisystemic wasting syndrome. Droplet digital polymerase chain reaction (ddPCR) is novel PCR method that can achieve high precision. Our study aimed to develop a sensitive assay utilizing ddPCR to detect PCV-2. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant...

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PMID: 26391713
DOI: 10.1177/1040638715608358

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