Article
Multiplex polymerase chain reaction for the detection and differentiation of 4 porcine circovirus 2 genotypes (PCV-2a, -2b, -2d, and -2e) in clinical samples.
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire - 1 Apr 2022
Park Kee Hwan, Cho Hyejean, Oh Taehwan, Yang Siyeon, Chae Chanhee
Abstract excerpt
The objective of this study was to develop multiplex polymerase chain reaction (PCR) for the simultaneous detection of porcine circovirus 2 (PCV-2) and differentiation among 4 PCV-2 genotypes (2a, 2b, 2d, and 2e) in collected clinical lymph node samples. The multiplex PCR detected each of 4 PCV-2 genotypes (2a, 2b, 2d, and 2e) to a dilution of 2 × 101 copies/μL. PCV-2a, PCV-2b, PCV-2d, and PCV-2e were propagated...
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