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Article

Development of a differential multiplex real-time PCR assay for porcine circovirus type 2 (PCV2) genotypes PCV2a, PCV2b and PCV2d.

Journal of virological methods - 1 Dec 2020

Wang Yin, Noll Lance, Porter Elizabeth, Stoy Colin, Dong Junsheng, Anderson Joe, Fu Jinping, Pogranichniy Roman, Woodworth Jason, Peddireddi Lalitha, Bai Jianfa

Abstract excerpt

A multiplex quantitative real-time polymerase chain reaction (mqPCR) assay was developed and validated for detection and differentiation of porcine circovirus type 2 (PCV2) genotypes, PCV2a, PCV2b and PCV2d. Single nucleotide polymorphism in primers or probes was deployed for different genotype detections, while conserved sequence in the 3' end of a primer and in the middle of a probe was used for the targeted...

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Identifiers

PMID: 32926893
DOI: 10.1016/j.jviromet.2020.113971
PMCID: PMC7486290

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